A nice puzzle but I'm surprised no one has asked for any help with it.
I have ended up with five thematic entries when there should be only four. The problem is with a repetition in 10 and 19D. However, I have two thematic letters in 39 - but the preamble says there's only one in each location.
Also, a clue I can't parse is 25: 'Bit of circuitry involved in recognising letter shapes in the eye' (6). The last three words are the definition, the bit must be 'c' and there's a thematic letter, which would be omitted from the wordplay. That leaves four letters that don't seem to lead to 'recognising letter shapes'.
It's late in the week to be asking for help, I know.