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Help please
Only three left to do
24across. A small volume injected in climber as protection. 7 letters n. - - - I. - e

27across. quiet and oddly eerie around midnight when moon's nearest. 7 letters p. - - - g. - e

25 down estate, for instance, is about right. 3 letters

I think 21 down is run up - clue is accelerate very short cryptic clue

27 Perigee

24 Vaccine

25 Car ?

Rev up.

Car is correct.

Thanks a lot