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Now, I'm going to regret this, but am stuck on last two, and I feel I ought to get them....
20d Protein, one very much unknown, with unknown mass and energy (7) I---E-E
and 25a Like an action replay? Look in two seconds (3-2) S--/-O

I think energy must be the definition of the first one, and the second one must be "see to" or someting.
As ever, all help welcome.

isozyme?

20d i so z y m e

25a slo mo

slo mo?

Are you sure of the first e?
One - i ,very -so, unknown- z, with unknown- y , mass and energy - me = isozyme, a protein?

Many thanks both. I should have got the protein thing being a trained biochemist and all that. pity I didnt take more care with the english!! Adverbs, eh?